Estudio comparativo de la detección de SARS CoV-2 por RT-PCR en muestras de hisopado nasofaringeo y saliva un estudio piloto en Bolivia
Estudio Comparativo de la Detección De Sars Cov-2 por Rt-Pcr En Muestras de Hisopado Nasofaringeo y Saliva
DOI:
https://doi.org/10.47993/gmb.v45i2.538Palabras clave:
COVID-19, saliva, SARS, prueba de laboratorio, reacción en cadena de la polimerasa de transcriptasa inversaResumen
Objetivos: El muestreo de hisopado nasofaríngeo para la detección de SARS CoV-2 es un método estándar para el diagnóstico de COVID-19, pero su recolección generalmente ocasiona incomodidad en el paciente y expone a un mayor riesgo al personal de salud. La muestra de saliva parece ser una buena alternativa con respecto a las muestras de hisopado nasofaringeo, no es invasiva, reduce el riesgo de contaminación del personal sanitario y permite la auto recolección. Este estudio tiene por objetivo comparar la capacidad de detectar al SARS CoV-2 por rRT-PCR en un mismo paciente, a partir de muestras de saliva y de hisopado nasofaríngeo para analizar la concordancia de los resultados obtenidos entre ambas muestras.
Métodos: Treinta muestras de saliva y de HNP de pacientes con síntomas de COVID-19 que ingresaron al servicio de emergencia del Hospital Clínico Viedma fueron tomadas en paralelo. Ambas muestras fueron analizadas por rRT-PCR para la detección de SARS CoV-2. La concordancia de resultados fue calculada por el coeficiente de kappa de Cohen.
Resultados: Nuestros resultados muestran que existe una buena concordancia (Índice Kappa 0,730; IC del 95%: 0,486 – 0,974) entre los dos tipos de muestras analizadas.
Conclusiones: La saliva parece ser una muestra fiable y efectiva para la detección del SARS CoV-2.
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